Anti-Frizzled 5 Antibody

SKU: orb737324

Description

Rabbit polyclonal antibody to FZD5

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Neuroscience,Signaling Pathways,Product Categories/Products/Antibodies/Primary Antibodies,Signaling Pathways/Wnt,Signaling Pathways/GPCR,Epigenetics,Developmental Biology

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1/500 - 1/1000, IF: 1/50 - 1/200
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Clone No.: FZD5; FZD8
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-FZD5 antibody, anti-C2orf31 antibody, anti-Frizzled-5 antibody, anti-Fz-5 antibody, anti-hFz5 antibody, anti-FzE5 antibody, anti-FZD8 antibody, anti-Frizzled-8 antibody, anti-Fz-8 antibody, anti-hFz8 antibody

Western blot analysis of Frizzled 5 expression in SGC7901 (A), PC3 (B), MEF (C) whole cell lysates.

Immunofluorescent analysis of Frizzled 5 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

UniProt

Q13467