NSE/ENO2 Antibody

SKU: orb443177

Description

Anti-NSE/ENO2 Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 5μg/ml Flow Cytometry (Fixed), 1-3μg/1x106 cells. Add 0.2ml of distilled water will yield a concentration of 500ug/ml

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human NSE, which shares 95.1% and 100% amino acid (aa) sequence identity with mouse and rat NSE, respectively.
• Molecular Weight: 47 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE; ENO2

Quick Database Links

UniProt

P09104

Valeria Manzini 1 2, Pamela Cappelletti 3, Nicola S Orefice 4 5 6, Ilaria Brentari 7, Michael J Rigby 4 5 8 9, Maria Lo Giudice 10, Marco Feligioni 3 11, Roberto Rivabene 1, Alessio Crestini 1, Francesco Manfredi 12, Giuseppina Talarico 13, Giuseppe Bruno 13, Massimo Corbo 3, Luigi Puglielli 4 5 14, Michela A Denti 15, Paola Piscopo miR-92a-3p and miR-320a are Upregulated in Plasma Neuron-Derived Extracellular Vesicles of Patients with Frontotemporal Dementia Mol Neurobiol, (2024)

PubMed: 39138758

Applications

WB

Reactivity

Human