CD44v10 Rabbit Polyclonal Antibody

SKU: orb5800

Description

CD44v10 Rabbit Polyclonal Antibody

Research Area

CD Markers, Cancer Research, CD Markers, Immune System, Immunology, Stem Cell Research, Stem Cells, Surface Molecules, Molecular Biology, Tumor Biomarkers, Cancer Research

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD44v10 (218-223+472-477/742, 223=T -> Naa)
• Target: CD44
• Molecular Weight: 52/82 kDa
• Purification: affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III

Blank control (blue line): U251 (fixed with 70% ethanol overnight at 4°C and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-CD44v10 antibody (orb5800), dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: Huvec (Human) Cell Lysate at 30 ug, Lane 3: A549 (Human) Cell Lysate at 30 ug, Lane 4: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-CD44v10 (orb5800) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 110-180 kD, Observed band size: 110-180 kD.

Quick Database Links

Gene Symbol

CD44

UniProt

P16070

Aoshiba, Kazutetsu et al. Senescence-associated secretory phenotype in a mouse model of bleomycin-induced lung injury Exp Toxicol Pathol, 65, 1053-1062 (2013)

PubMed: 23688655

Applications

IHC

Reactivity

Mouse

Tei, Hiromoto et al. Expression profile of CD44s, CD44v6, and CD44v10 in localized prostate cancer: effect on prognostic outcomes following radical prostatectomy Urol Oncol, 32, 694-700 (2014)

PubMed: 24468288

Applications

IHC

Reactivity

Mouse