Anti-betaIII-Tubulin Purified

SKU: orb44544

This product can be custom made

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Description

Mouse monoclonal antibody to Tubulin beta 3

Research Area

Epigenetics

Images & Validation

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Item 1 of 6

Tested Applications	FC, ICC, IHC-P, WB
Reactivity	Canine, Human, Mouse, Porcine, Rat
Application Notes	Western blotting: Recommended dilution: 1-2 μg/ml; positive control: porcine brain lysate; negative control: HPB-ALL human peripheral blood leukemia cell line; reducing conditions. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml, standard ABC technique (DAB+), pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or high temperature citrate buffer antigen retrieval; positive tissue: neuronal tissue. Immunocytochemistry: Positive material: Neuro2a mouse neuroblastoma cell line. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG1
Clone No.	TU-20
Immunogen	Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
Target	betaIII-Tubulin
Purity	Purified by protein-A affinity chromatography.
Purification	Purified by protein-A affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti-beta 3 tubulin antibody, anti-beta-4 antibody, anti-CDCBM antibody, anti-CFEOM3A antibody, anti-M(beta)3 antibody, anti-M(beta)6 antibody, anti-MC1R antibody, anti-Neuron specific beta III Tubulin antibody, anti-Neuron-specific class III beta-tubulin antibody, anti-Tubb 3 antibody, anti-TUBB3 antibody, anti-TUBB4 antibody, anti-Tubulin beta 3 antibody, anti-Tubulin beta 3 chain antibody, anti-Tubulin beta 4 antibody, anti-Tubulin beta III antibody, anti-Tubulin beta-3 chain antibody, anti-Tubulin beta-4 chain antibody, anti-Tubulin beta-III antibody

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