Blotto Immunoanalytical Grade (Non-Fat Dry Milk)

SKU: orb348624

Description

Blotto Immunoanalytical Grade (Non-Fat Dry Milk)

Research Area

Product Categories/Research Tools,Blocker Type: BLOTTO

Images & Validation

• Application Notes:
  Use Rockland's BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk. Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available. BLOTTO A is a convenient general purpose blocking agent. BLOTTO B is recommended for blocking when phospho-specific antibodies are used. BLOTTO A and BLOTTO B provide Tween-20 in a separate vial in liquid form (p/n TW0025). Users should consider the addition of protease inhibitors and phosphatase inhibitors when required. Typically 50 mM NaF can be added to BLOTTO solutions to inhibit phosphatase activity.

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Key Properties

• Purity: This product contains highly purified immunoanalytical grade non-fat dry milk.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store container at room temperature prior to opening. After reconstitution, use blocking buffers immediately. Dilute solutions may be stored at 4° C for up to four (4) days. Solutions containing BLOTTO may be frozen.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: None. Stabilizer: None. None
• Expiration Date: 12 months from date of receipt.
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

Blotto Immunoanalytical Grade Non-Fat Dry Milk, Blocking reagent for western, antibody dilution buffer