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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human B7-2, His Tag at 2ug/mL (100 ul/well),can bind Human CD28, Fc Tag with a linear of 0.01-5 ug/mL. |
| Target | CD86 |
| Tag | His Tag |
| MW | 26.7 kDa |
| Purity | > 95% as determined by SDS-PAGE. |
| Protein Sequence | Leu 26 - Pro 247 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human B7-2 Protein, mFc-His Tag [orb689395]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 52.4 kDa after removal of the signal peptide.
Mammalian
100 μg, 10 μg, 50 μgRecombinant human CD86 / B7-2 protein, C-His-Avi (HEK293) [orb1516532]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. kDa
50 μg, 100 μgMouse B7-2 Protein, hFc Tag [orb1290895]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 51.4 kDa after removal of the signal peptide. The apparent molecular mass of mB7-2-hFc is approximately 55-70 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg

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