Dog Cross Linked C-Telopeptide of Type III Collagen (CTXIII) ELISA Kit

SKU: orb782390

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cross Linked C-Telopeptide Of Type III Collagen(CTXIII) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cross Linked C-Telopeptide Of Type III Collagen(CTXIII).

Research Area

Metabolism Research, Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog CTXIII protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog CTXIII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog CTXIII in the samples is then determined by comparing the OD of the samples to the standard curve

  Read more...

Key Properties

• Reactivity: Canine
• Sample Types: Serum, plasma and other biological fluids
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 156.25-10000 pg/mL
• Sensitivity: 45.8 pg/mL
• Concentration: 10000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

CTX-III ELISA Kit, CTX3 ELISA Kit