F(ab')2 Mouse IgG (H&L) Antibody Fluorescein Conjugated

SKU: orb348245

Description

F(ab')2 Mouse IgG (H&L) antibody (FITC)

Research Area

Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: ELISA, FC, FLISA, IF, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:100,000, FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IHC: 1:1,000 - 1:5,000, IF: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Mouse
• Application Notes:
  F(ab')2 Mouse IgG (H&L) Antibody Fluorescein Conjugated has been tested by western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG F(ab')2
• Immunogen: Mouse IgG whole molecule
• Purity: F(ab')2 Mouse IgG (H&L) Antibody Fluorescein Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
• Conjugation: FITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Goat F(ab')2 Anti-Mouse IgG Antibody Fluorescein Conjugation, Goat Fab2 Anti-Mouse IgG FITC conjugated Antibody