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XRCC4-like factor/NHEJ1 Antibody

SKU: orb19337

Description

Goat polyclonal antibody to NHEJ1

Images & Validation

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Tested ApplicationsELISA, FC, IF, IHC, WB
Dilution RangeELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 2.5-3.8µg/ml
ReactivityHuman
Predicted ReactivityCanine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 38kDa band observed in lysates of Human Skin, Testis and Thyroid gland (calculated size of 33.3kDa according to NP_079058.1). The observed molecular weight corresponds to findings with antibodies from other sources. The 38kDa band was successfully blocked by incubation with the immunizing peptide. Recommended concentration 0.1-0.3 μg/ml.

Key Properties

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HostGoat
ClonalityPolyclonal
TargetXRCC4-like factor / NHEJ1
Protein SequenceQRPQLSKVKRKKPR
Molecular Weight33.3
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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anti NHEJ1 antibody, anti XRCC4-like factor antibody, anti nonhomologous end-joining factor 1 antibody, anti FLJ12610 antibody, anti XLF antibody, anti Cernunnos antibody

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

NCBI Gene Details

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No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 500.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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