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Human ASF1A protein

SKU: orb391261

Description

Recombinant human ASF1A protein is produced by our E. coli expression system and the target gene encoding Met1-Met204 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.

Research Area

Product Categories/Products/Proteins,Product Categories/Research Area,Product Categories/Proteins,Epigenetics

Images & Validation

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Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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SourceE. coli
TargetASF1A
ReactivityHuman
EndotoxinsEndotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
PurityGreater than 95% as determined by reducing SDS-PAGE.
Protein SequenceMASMTGGQQM GRGSMAKVQV NNVVVLDNPS PFYNPFQFEI TFECIEDLSE DLEWKIIYVG SAESEEYDQV LDSVLVGPVP AGRHMFVFQA DAPNPGLIPD ADAVGVTVVL ITCTYRGQEF IRVGYYVNNE YTETELRENP PVKPDFSKLQ RNILASNPRV TRFHINWEDN TEKLEDAESS NPNLQSLLST DALPSASKGW STSENSLNVM LESHMDCMLE HHHHHH

Storage & Handling

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StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μM filtered solution of 20mM Tris-HCl, 1mM DTT, 150mM NaCl, pH 8.0.
Buffer/PreservativesLyophilized from a 0.2 μM filtered solution of 20mM Tris-HCl, 1mM DTT, 150mM NaCl, pH 8.0.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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Histone chaperone ASF1A; Anti-silencing function protein 1 homolog A; hAsf1; hAsf1a; CCG1-interacting factor A; CIA; hCIA

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Quality Guarantee

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UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Available Sizes

Select a size below

10 μg
$ 340.00
50 μg
$ 910.00
500 μg
$ 3,390.00
DispatchUsually dispatched within 5-10 working days
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