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Human IL21 protein

SKU: orb80080

Description

Human IL21 protein

Images & Validation

Tested ApplicationsFA, HPLC, SDS-PAGE
Application Notes
< strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.54 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of IL-21 as Reference Standard.

Key Properties

Biological OriginE.coli
Biological ActivityThe ED50=1.0 -10 ng/ml, determined by the dose-dependant proliferation of human peripheral blood mononuclear cells.
TargetHuman IL21
ConjugationUnconjugated
Purity> 95.0% as determined by RP-HPLC and analysis by SDS-PAGE
Protein SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Gln-Gly-Gln-Asp-Arg.

Storage & Handling

StorageStore at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Buffer/PreservativesLyophilized from concentrated (1mg/ml) solution containing no additives.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

IL 21 protein, IL21 protein, IL-21 protein, Interleukin 21 protein, Interleukin21 protein, Za11 protein, Interleukin-21 protein, interleukin-21 isoform protein

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Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol
HPLC
High-Performance Liquid Chromatography
View Protocol

Available Sizes

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10 μg
$ 340.00
100 μg
$ 1,480.00
500 μg
$ 3,300.00
DispatchUsually dispatched within 2-3 weeks
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