Human LR3IGF1 protein

SKU: orb82618

Description

Insulin-like growth factor-1 (IGF1) is the principal hormonal mediator of statural growth. Under normal circumstances, growth hormone (GH) binds to its receptor in the liver, and other tissues, and stimulates the synthesis/secretion of IGF1. In target tissues, the Type 1 IGF receptor, which is homologous to the insulin receptor, is activated by IGF1, leading to intracellular signaling which stimulates multiple processes leading to statural growth. The metabolic actions of IGF1 are in part directed at stimulating the uptake of glucose, fatty acids, and amino acids so that metabolism supports growing tissues.

Images & Validation

• Tested Applications: ELISA, FA, HPLC, SDS-PAGE, WB
• Application Notes:
  Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Key Properties

• Biological Origin: E.coli
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the stimulation of protein synthesis in L6 myoblasts is less then 10 ng/ml. Less than 5EU/100μg of rHuLR3IGF1 as determined by LAL method.
• Target: IGF1 LONG R3
• Conjugation: Unconjugated
• MW: 9.1 kDa
• Purity: > 95% pure by SDS-PAGE and HPLC analyses.
• Protein Sequence: MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA

Storage & Handling

• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: Lyophilized from a 0.2 μm filtered concentrated (1mg/ml) solution in 20mM PB, pH 7.2, 4% mannitol. We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

IGF1 LONG R3