Integrin beta 3 Antibody / ITGB3 / CD61

SKU: orb385996

Description

Reacts with human CD61, also called Integrin beta 3 (GPIIIa, vitronectin receptor beta chain). It associates with the αV-chain (CD51) to form vitronectin receptor, or with the αIIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61). The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Infectious Diseases

Images & Validation

• Tested Applications: IHC
• Dilution Range: Immunohistochemistry (Frozen & FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Titration of the CD61 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: Y2/51
• Immunogen: Phytohemagglutinin (PHA)-stimulated peripheral blood cells were used as the immunogen for the CD61 antibody.
• Purity: Protein G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store the CD61 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This CD61 antibody is available for research use only.
• Disclaimer: For research use only

Quick Database Links

UniProt

P05106