mCherry-T2A-Luciferase mRNA-LNP

SKU: orb2719893

Description

mCherry is a protein of the monomeric red fluorescent protein (mRFP) mFruits family, derived from DsRed of the Discosoma sea anemone. mCherry is a fluorescent protein chromophore used as a tool to visualize genes and analyze their function in experiments. mCherry absorbs light in the range of 540-590 nm and emits light in the range of 550-650 nm. Luciferase is an oxidase that produces bioluminescence and is widely used in biotechnology as a reporter gene. Unlike fluorescent proteins, luciferase does not require an external light source, but requires the addition of the substrate luciferin. This product is designed as a tool for the delivery and expression of both mCherry and Luciferase using mCherry-t2A-Luciferase mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of mCherry-t2A-Luciferase mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. mCherry-t2A-Luciferase mRNA-LNP produces mCherry and Luciferase separately by a T2A self-cleaving peptide. The sequence of mCherry protein consists of 236 amino acids, and the GenPept accession number is WIO52923. The sequence of luciferase protein consists of 550 amino acids, and the GenPept accession number is AAA89088.

Images & Validation

• Application Notes:
  Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.

  Read more...

Storage & Handling

• Storage: 4°C; ice
• Form/Appearance: mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only