Metformin

SKU: orb1298860

Description

Metformin

Research Area

Product Categories/Products/Small Molecules,Autophagy|||Chromatin/Epigenetic|||PI3K/Akt/mTOR signaling

Key Properties

• CAS Number: 657-24-9
• MW: 129.16
• Purity: 99.74%
• Formula: C₄H₁₂ClN₅
• SMILES: CN(C)C(=N)NC(N)=N

Bioactivity

• Target IC50:
  TNF-α:3.35 μM|PANC1 cells viability:20.95 ± 0.98 mM|B-CPAP cells viability:5.329 mM|SARS-CoV-2 (Caco2 cells):1.43 mM|SARS-CoV-2 (Calu3 cells):0.4 mM
• In Vivo:
  METHODS: To model Metformin-induced diarrhea, Metformin (125-500 mg/kg) was administered orally to healthy and diabetic obese db/db C57BL/6J mice twice daily for thirteen days. RESULTS: Metformin at 1000 mg/kg/day significantly increased fecal water content. Although no diarrhea symptoms were observed in healthy C57BL/6J mice, the same dose of Metformin induced severe diarrhea in diabetic obese db/db mice. METHODS: To investigate the protective effect of Metformin in radiation injury, Metformin (200 mg/kg once daily for three days) was administered orally to BALB/c mice, which were then exposed to 6-8 Gy of gamma radiation. RESULTS: When administered prior to exposure to radiation, Metformin prolonged the survival of mice exposed to 8 Gy-TBI and increased the survival of mice exposed to 6 Gy-TBI. Pretreatment with Metformin attenuated radiation damage.
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• In Vitro:
  METHODS: Ovarian cancer cells A2780 and SKOV3 were treated with Metformin (0.001-50 mM) for 24-48 h. Cell viability was assayed using the MTS RESULTS: Micromolar concentrations of Metformin did not statistically reduce the viability of the A2780 or SKOV3 cell lines. At 48 h, millimolar concentrations resulted in cell death. METHODS: Human colorectal cancer cells HCT29 were treated with Metformin (0.6 mM) for 90 h. Cell motility was detected using the wound healing assay and chamber invasion assay. RESULTS: Metformin inhibited the migration and invasion of HCT29 cells, and Metformin decreased the motility of tumor cells.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Compound Identifiers

PubChem CID

152743144