Mouse Kallikrein 1 (KLK1) ELISA Kit

SKU: orb2667699

Description

Mouse Kallikrein 1 (KLK1) ELISA Kit

Research Area

Cancer Biology, Gastroenterology & Hepatology, Protein Biochemistry

Images & Validation

• Application Notes:
  standard: 20 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse KLK1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KLK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KLK1 in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Sample Types: Serum, plasma and other biological fluids.
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.14 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

KLKR; Klk6; HK1; Kallikrein 1,Renal/Pancreas/Salivary; Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein

Quick Database Links

UniProt

P15947