NF-kB (phospho-65) ELISA Kit

SKU: orb343760

Description

NF-kB (phospho-65) ELISA Kit

Research Area

Product Categories/Products/ELISA Kits,Detection Kit Type: ELISA Kit

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  Rockland's NF-kB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kB response element is immobilized onto the bottom of wells of a 96 well plate (see Figure 1 on page 4). NF-kB contained in a nuclear extract specifically binds to the NF-kB response element. NF-kB (p65) is detected by addition of a specific primary antibody directed against NF-kB (p65). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. Rockland's NF-kB (p65) Transcription Factor Assay detects human NF-kB (p65). It will not cross-react with NF-kB (p50).

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Key Properties

• Reactivity: Human
• Conjugation: Unconjugated

Storage & Handling

• Storage: See kit insert for complete instructions.
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

NF-kB Transcription Factor Kit, p65 kit, EIA kit, NF-kB p65 Transcription Factor Assay