pCp-Cy5

SKU: orb63300

Description

pCp-Cy5 is recommended for 3'-End Labeling of RNA/miRNA with T4 RNA Ligase that conjugates a single dye molecule to the 3'-OH group of the RNA/miRNA template. The resulting Dye-labeled RNA/miRNA probes are ideally suited for hybridization experiments such as microarray-based expression profiling or electro mobility shift assays (EMSA).

Images & Validation

• Application Notes:
  Incorporation into RNA/miRNA by 3'-End Labeling with T4 RNA Ligase. ; Spectroscopic Propertie: λexc 649 nm, λem 670 nm, ε 250.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

Key Properties

• MW: Theoretical MW: 1141.14 g/mol (free acid); Detected MW: 1140.34 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₄₈H₆₆N₆O₁₈P₂S₂
• SMILES: C(=C\C1=*(CC)c2c(C1(C)C)cc(S(=O)(=O)[O-])cc2)/C=C/C=C\1/N(c2c(C1(C)C)cc(cc2)S(=O)(=O)O)CCCCCC(=O)NCCCCCCOP(=O)(O[C@@H]1[C@@H](COP(=O)(O)O)O[C@@H](n2c(=O)nc(N)cc2)[C@@H]1O)O

Storage & Handling

• Storage: store at -20 °C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only