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Description
Images & Validation
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| Tested Applications | ELISA, MS, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | E. coli |
|---|---|
| Target | BDNF |
| Reactivity | Rat |
| Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
| Protein Sequence | MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 250mM NaCl, pH 7.2. |
| Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 250mM NaCl, pH 7.2. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Brain-derived neurotrophic factor; BDNF; Abrineurin
Similar Products
−Human/Murine/Rat BDNF Protein [orb1471779]
Greater than 95% as determined by reducing SDS-PAGE.
13 KDa
Mammalian
10 μg, 50 μgRat BDNF protein [orb245654]
Greater than 90% as determined by SDS-PAGE.
16.3 kDa
E.coli
20 μg, 100 μg, 1 mgRecombinant Human Neurotrophin-4 (rHuNT-4 ) [orb1495027]
> 97% by SDS-PAGE and HPLC analyses.
28 kDa, a noncovalently linked homodimer of two 14.0 kDa polypeptide monomers (260 total amino acid residues).
Escherichia coli.
1 mg, 10 μg, 2 μgBDNF Protein, Human/Murine/Rat, Recombinant [orb1962089]
> 99.9%
14 KDa (reducing condition)
500 μg, 1 mg, 10 μg, 50 μg

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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
