Rat IgG1 isotype control Phycoerythrin Antibody

SKU: orb343824

Description

Rat IgG1 isotype control Phycoerythrin Antibody

Research Area

Product Categories/Products/Antibodies,PSC Type: Protein

Images & Validation

• Tested Applications: DOT, ELISA, FC
• Application Notes:
  RAT IgG1 isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.

Key Properties

• Source: Rat
• Biological Origin: Rat
• Isotype: IgG1
• Conjugation: RPE
• Purity: RAT IgG1 isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-phycoerythrin, anti-Rat IgG and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG2a, IgG2b, IgG3, IgM, and IgA. Phycoerythrin Conjugated Rat IgG1 confirmed by Dot Blot.

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 0.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rat Isotype Control, Rat IgG1 PE isotype, RAT IgG1 subclass isotype control