Taq-Polymerase RecAb (TP7)

SKU: orb2568780

Description

Taq-Polymerase RecAb (TP7) is a recombinant mouse antibody (mIgG2a,κ) that binds to Taq polymerase and inhibits the polymerase activity at room temperature. The antibody is inactivated and the enzyme released upon temperature increase (“hot start antibody”) during normal PCR cycling conditions, allowing reactions to be set up at room temperature. The target Taq polymerase (Uniprot: P19821) is produced by the thermophilic eubacterium Thermus aquaticus and is a popular thermostable DNA polymerase used for PCR [1][2][3]. The original TP7 antibody was prepared by Scalice et al. (1994) [4] through mouse immunization with recombinant Taq expressed in E. coli. The recombinant antibody is produced under serum‐free (animal component-free) conditions in CHO cells system and purified through one-step purification with Protein-A affinity chromatography. We recommended to use TP7 antibody at a ≥ 1:1 molar ratio over Taq polymerase.

Images & Validation

• Tested Applications: PCR
• Reactivity: Bacteria

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a κ
• Clone No.: TP7
• Immunogen: recombinant TaqPol expressed in E. coli
• Target: Taq-Polymerase
• Purity: ≥ 90% (CGE, reducing conditions). ≤ 10 % aggregates (analytical SEC)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Clear Liquid, PBS, pH 7.4, 0.2 μm sterile filtered
• Concentration: ≥ 0.5 mg/ mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA polymerase I, thermostable, polA, pol1

Quick Database Links

Gene Symbol

Taq-Polymerase

UniProt

P19821