2-Ethynyl-adenosine

SKU: orb154335

Description

2-Ethynyl-labeled adenosine (2-EA) can be used to measure de novo mRNA poly(A) tail synthesis in proliferating cells. 2-EA is cell permeable and incorporates into nascent mRNA transcripts both transcriptionally by RNA polymerase I, II and III and posttranscription-ally by poly(A) polymerase instead of their natural analolg adenosine. The resulting ethynyl-functionalized RNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging.

Images & Validation

• Application Notes:
  mRNA poly(A) tail synthesis monitoring.

Key Properties

• MW: Theoretical MW: 291.26 g/mol; Detected MW: 291.10 g/mol
• Purity: 95% (HPLC)
• Formula: C₁₂H₁₃N₅O₄
• SMILES: c12c(nc(nc2n(cn1)[C@H]1[C@@H]([C@@H]([C@H](O1)CO)O)O)C#C)N
• Solubility: up to 4.0 mM in 1x PBS

Storage & Handling

• Storage: store at -20 C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only