2-Ethynyl-ATP (2-EATP)

SKU: orb1734887

Description

2-Ethynyl-labeled adenosine triphosphate (2-EATP) is suitable for in vitropolyadenylation of RNA with recombinant poly(A) polymerase. The resulting Alkyne-functionalized RNA can subsequently be processed via Cu(I)-catalyzed Azide-Alkyne click chemistry (CUAAC) that offers the choice: to introduce a Biotin group for subsequent purification tasks (via Azides of Biotin); to introduce fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes); to crosslink the RNA to azide-functionalized biomolecules e.g.proteins.

Images & Validation

• Application Notes:
  in vitro polyadenylation of RNA. < br>< b>Spectroscopic Propertie: λmax 265 nm, ε 10.6 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• Molecular Weight: Theoretical MW: 531.20 g/mol; Detected MW: 531.00 g/mol
• Purity: ≥ 90% (HPLC), contains approx. 6% 2-Ethynyl-ADP
• Formula: C₁₂H₁₆N₅O₁₃P₃
• SMILES: [C@@H]1([C@@H](O[C@@H]([C@H]1O)COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1cnc2c(nc(nc12)C#C)N)O

Storage & Handling

• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Form/Appearance: solution in 100 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
• Concentration: 10 mM-11 mM
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Compound Identifiers

PubChem CID

122377602