A-Raf rabbit pAb

SKU: orb764568

Description

Rabbit polyclonal antibody to A-Raf

Research Area

Product Categories/Antibodies/Primary Antibodies,Product Categories/Small Molecules,Product Categories/Antibodies

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution Range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human A-RAF. AA range:276-325
• Molecular Weight: 68
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-ARAF antibody, anti-ARAF1 antibody, anti-PKS antibody, anti-PKS2 antibody, anti-Serine/threonine-protein kinase A-Raf antibody, anti-Proto-oncogene A-Raf antibody, anti-Proto-oncogene A-Raf-1 antibody, anti-Proto-oncogene Pks antibody

Western blot analysis in MCF-7 cells transfected with siA-Raf. Cancer Letters 355 (2014) 288–296.

Western Blot analysis of various cells using A-Raf Polyclonal Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using A-RAF Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HeLa cells, treated with PMA 125ng/ml 30', using A-RAF Antibody. The lane on the right is blocked with the synthesized peptide.