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ACHE Antibody

SKU: orb1248236

Description

ACHE Antibody

Research Area

,Cancer

Images & Validation

Tested ApplicationsELISA, FC, IF, WB
ReactivityHuman, Rat
Predicted ReactivityMouse
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in HepG2 and Jurkat cell lysates and approx. 65-70kDa in Daudi and HeLa cell lysates (calculated MW of 67.8kDa according to NP_000656.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Key Properties

Antibody TypePrimary Antibody
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: QFDHYSKQDRCSDL
TargetACHE
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

ACHE, YT, acetylcholinesterase (YT blood group), ARACHE, N-ACHE, OTTHUMP00000211347, OTTHUMP00000211349, OTTHUMP00000211356, acetylcholinesterase, apoptosis-related acetylcholinesterase

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 880.00
Free Secondary Antibody (20 ul)0/0

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