ACTA1/α-actin Antibody (C-term)

SKU: orb1788392

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Primary Antibodies;Stem Cells;Anti-Wnt-Pathway (plus Lgr5) Antibodies;Inflammation mediated by chemokine and cytokine signaling

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB: 1:1000, IHC: 1:25
• Reactivity: Human, Rat
• Predicted Reactivity: C. elegans, Drosophila, Equine, Hamster, Mouse, Other, Porcine, Rabbit, Sheep, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: 189-217 aa
• Target: This ACTA1/Alpha-actin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-217 amino acids from the C-terminal region of human ACTA1/Alpha-actin.
• Molecular Weight: 42051 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ACTA1; ACTA; Actin, alpha skeletal muscle; Alpha-actin-1

Western blot analysis of lysates from human normal uterus, human placenta, rat heart tissue lysate (from left to right), using ACTA1/α-actin (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Staining ACTA1/alpha-actin in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

This ACTA1/Alpha-actin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-217 amino acids from the C-terminal region of human ACTA1/Alpha-actin.

UniProt

P68133

RefSeq (Protein):NP_001091.1