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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human Actin alpha 1. |
| Target | ACTN1 |
| Molecular Weight | 103 kDa |
| Purification | Antibody is purified by peptide affinity chromatography method. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Antibody used in WB on Rat Cortical Neurons at 1:1000.

Antibody used in WB on Human 293T at 0.2-1 ug/ml.

Antibody used in WB on Human HEK293 at 1:1000.

Antibody used in WB on rACTNX-GFP at 1:1000 (Lane 1: 10 ug ACTN1-GFP transfected COS-7 lysate, Lane 2: 10 ug ACTN2-GFP transfected COS-7 lysate, Lane 3: 10 ug ACTN3-GFP transfected COS-7 lysate, Lane 4: 10 ug ACTN4-GFP transfected COS-7 lysate).

Antibody used in IHC on rACTNX-GFP at 1:1000.
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Protocol Information
Actin alpha 1 Antibody (orb1244248)
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