ADAR Antibody

SKU: orb1256130

Description

ADAR Antibody

Research Area

,Other

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human ADAR (NP_001102.2).
• Target: ADAR
• Molecular Weight: Observed: 136kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ADAR1, AGS6, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88DSRBP, P136, double-stranded RNA-specific adenosine deaminase, 136 kDa double-stranded RNA-binding protein, adenosine deaminase acting on RNA 1-A, dsRNA adenosine deaminase, dsRNA adeonosine deaminase, interferon-induced protein 4, interferon-inducible protein 4

Western blot analysis of extracts of various cell lines, using ADAR antibody (orb1256130) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 20s.

Immunohistochemistry of paraffin-embedded rat testis using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat heart using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 3 ug SUZ12 antibody (orb1274421). Western blot was performed from the immunoprecipitate using SUZ12 antibody (orb1274421) at a dilution of 1:500.

Immunofluorescence analysis of HeLa cells using ADAR antibody (orb1256130) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

ADAR

UniProt

P55265