ADRB2 Rabbit Polyclonal Antibody

SKU: orb10056

Description

ADRB2 Rabbit Polyclonal Antibody

Research Area

Cardiovascular Research, G Protein-Coupled Receptors, GPCR Signaling Pathway, Myocardial regeneration, Cardiac

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ADRB2 (201-300/418aa)
• Target: ADRB2
• Molecular Weight: 46 kDa
• Purification: affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

beta 2-adrenergic receptor; beta 2 Adrenergic Receptor; ADRB2R; ADRBR; ADRB2_HUMAN; Adrenergic beta 2 receptor surface; B2AR; BAR; beta 2 adrenoreceptor; BETA2AR; Catecholamine receptor; beta2-adrenergic receptor.

Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ADRB2) Polyclonal Antibody, Unconjugated (orb10056) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Positive control: (mo) Splenocytes (2% Paraformaldehyde-fixed), Isotype Control Antibody, Antibody: Rabbit IgG, Dilution: 1 µg in 100 µl 1 X PBS containing 0.5% BSA, Secondary Antibody, Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:200 in 1 X PBS containing 0.5% BSA, Primary Antibody, catalog number: orb10056, Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA.

Sample: Lane 1: Mouse Heart tissue lysates, Lane 2: Mouse Stomach tissue lysates, Lane 3: Mouse Cerebrum tissue lysates, Lane 4: Rat Heart tissue lysates, Lane 5: Rat Stomach tissue lysates, Lane 6: Rat Cerebrum tissue lysates, Lane 7: Human A431 cell lysates, Primary: Anti-TBX1 (orb10056) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46 kDa, Observed band size: 50 kDa.

Quick Database Links

Gene Symbol

ADRB2

UniProt

P07550