AGO3 Antibody / Argonaute-3 / eIF2C3

SKU: orb2636539

Description

This antigen is involved in binding and translation repression of short RNAs. This antigen is also known as Argonaute RISC catalytic component 3. Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5-flanking sequences to the region of guide-target complementarity.

Images & Validation

• Tested Applications: ELISA, FACS, IF
• Dilution Range: ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the AGO3 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: PCRP-AGO3-1C5
• Immunogen: Recombinant full-length human AGO3 protein was used as the immunogen for the AGO3 antibody.
• Purity: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This AGO3 antibody is available for research use only.
• Disclaimer: For research use only

Quick Database Links

UniProt

Q9H9G7