AIF Antibody, KO Validated

SKU: orb1274462

Description

AIF Antibody, KO Validated

Research Area

,Apoptosis,Cell Cycle

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 334-613 of human AIF (NP_004199.1).
• Target: AIFM1
• Molecular Weight: Observed: 67kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AIF Antibody: CNK, KSR, CNK1, Connector enhancer of kinase suppressor of ras 1, CNK homolog protein 1, Connector enhancer of KSR 1

Western blot analysis of extracts of various cell lines, using AIF antibody (orb1274462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts from normal (control) and AIF knockout (KO) 293T cells, using AIF antibody (orb1274462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat lung using AIF antibody (orb1274462) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using AIF antibody (orb1274462) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using AIF antibody (orb1274462) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using AIF antibody (orb1274462) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using AIF antibody (orb1274462). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using AIF antibody (orb1274462) at dilution of 1:200 (60x lens). Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of Hela cells using AIF antibody (orb1274462) at dilution of 1:200. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

AIFM1

UniProt

O95831