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Description
Research Area
Akt/PKB Signaling Pathway, Apoptosis, Cell Cycle, Metabolism, Nuclear Signaling Pathway, PI3K/AKT Signaling Pathway
Images & Validation
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Item 1 of 2
| Tested Applications | ICC, WB |
|---|---|
| Dilution Range | WB=1:500-1000, ICC/IF=1:50-500 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | 10D8 |
| Immunogen | KLH conjugated synthetic peptide derived from human AKT (420-479/479aa) |
| Target | AKT1 |
| Molecular Weight | 56 kDa |
| Purification | affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−AKT 1; AKT; AKT1; AKT-1; AKT1_HUMAN; C AKT; cAKT; MGC9965; MGC99656; Oncogene AKT1; PKB; PKB alpha; PKB-ALPHA; PRKBA; Protein Kinase B alpha; Protein kinase B; Proto-oncogene c-Akt; RAC alpha; RAC alpha serine/threonine protein kinase; RAC; RAC PK alpha; Rac protein kinase alpha; RAC Serine/Threonine Protein Kinase; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; v akt murine thymoma viral oncogene homolog 1; vAKT Murine Thymoma Viral Oncogene Homolog 1.
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Quick Database Links
Gene Symbol
AKT1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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