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Description
Research Area
Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Neuroscience,Signaling Pathways,Signaling Pathways/Apoptotic,Epigenetics,Developmental Biology,Phosphoinosotode Signaling,PI3K AKT signaling,Protein Kinase C Signaling,Immune Cell Signaling,Warburg Effect,Translational Contral elF4 and p70 S6 Kinase,Regulation of Microtubule Dynamics,T cell receptor signaling,Inhibition of Apoptosis,Insulin &Glucose Signaling,Cell Cycle Control G1 S Checkpoint,Adherens Junction signaling,Mitochondrial Control of Apoptosis,MTOR signaling,ErbB HER Signaling,
Images & Validation
−
| Tested Applications | IF, IP, WB |
|---|---|
| Dilution Range | WB: 1:5000~1:10000, IF: 1:50~1:200, IP: 1:50~1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Synthetic phosphopeptide derived from human Akt around the phosphorylation site of Serine 473. |
| Molecular Weight | ~ 60 kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB, RAC
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
IP
Immunoprecipitation


















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