AKT1 monoclonal antibody (M03), clone 2E11

SKU: orb2296237

Description

Mouse monoclonal antibody raised against a full length recombinant AKT1.

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, IP, WB
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a Kappa
• Clone No.: 2E11
• Immunogen: AKT1 (AAH00479.1, 1 a.a. ~ 480 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTLHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

AKT1 monoclonal antibody (M03), clone 2E11 Western Blot analysis of AKT1 expression in HeLa.

AKT1 monoclonal antibody (M03), clone 2E11. Western Blot analysis of AKT1 expression in HepG2.

AKT1 monoclonal antibody (M03), clone 2E11. Western Blot analysis of AKT1 expression in MCF-7.

AKT1 monoclonal antibody (M03), clone 2E11. Western Blot analysis of AKT1 expression in NIH/3T3.

AKT1 monoclonal antibody (M03), clone 2E11. Western Blot analysis of AKT1 expression in PC-12.

AKT1 monoclonal antibody (M03), clone 2E11. Western Blot analysis of AKT1 expression in Raw 264.7.

Detection limit for recombinant GST tagged AKT1 is approximately 3 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell. [antibody concentration 20 ug/ml]

Immunoperoxidase of monoclonal antibody to AKT1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]

Immunoprecipitation of AKT1 transfected lysate using anti-AKT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AKT1 MaxPab rabbit polyclonal antibody.

Western Blot analysis of AKT1 expression in transfected 293T cell line by AKT1 monoclonal antibody (M03), clone 2E11. Lane 1: AKT1 transfected lysate(55.7 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (78.54 KDa).

Quick Database Links

RefSeq:AAH00479.1