AKT1 monoclonal antibody (M09), clone 6F11

SKU: orb2296231

This product can be custom made

Description

Mouse monoclonal antibody raised against a full length recombinant AKT1.

Images & Validation

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Item 1 of 8

Tested Applications	ELISA, IF, WB
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2a Lambda
Clone No.	6F11
Immunogen	AKT1 (AAH00479.1, 1 a.a. ~ 480 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Protein Sequence	MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTLHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	In 1x PBS, pH 7.4
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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AKT1 monoclonal antibody (M09), clone 6F11 Western Blot analysis of AKT1 expression in Jurkat.

AKT1 monoclonal antibody (M09), clone 6F11. Western Blot analysis of AKT1 expression in NIH/3T3.

AKT1 monoclonal antibody (M09), clone 6F11. Western Blot analysis of AKT1 expression in PC-12.

AKT1 monoclonal antibody (M09), clone 6F11. Western Blot analysis of AKT1 expression in Raw 264.7.

Detection limit for recombinant GST tagged AKT1 is 0.3 ng/ml as a capture antibody.

Detection limit for recombinant GST tagged AKT1 is approximately 10 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell. [antibody concentration 10 ug/ml]

Western Blot detection against Immunogen (78.54 KDa).

Quick Database Links

RefSeq:AAH00479.1

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	AAH00479.1

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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