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AKT1 (Prognostic Marker for Neuroendocrine Tumors) Antibody

SKU: orb2317392

Description

AKT1 (Prognostic Marker for Neuroendocrine Tumors) Antibody

Research Area

,AKT Signaling|Apoptosis|Autophagy|BBB VCAM-1 Signaling|Breast Cancer|Cardiovascular|Colon Cancer|Cytokine Signaling|Developmental Biology|Hypoxia|Immuno Oncology|Immunology|Infectious Disease|Lung Cancer|MAPK Signaling|Neuroinflammation|Nuclear Marker|Ovarian Cancer|Signal Transduction|Transcription Factors

Images & Validation

Tested ApplicationsIHC, WB
Dilution RangeWestern Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
ReactivityHuman
Application Notes
Positive Control: MCF-7 cells. PDGF-treated NIH/3T3 cells. HeLa cell lysates. Human pancreas or cervical carcinoma

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG2b
Clone No.AKT1/2491
ImmunogenRecombinant fragment of human AKT1 protein (around aa 85-189) (exact sequence is proprietary)
TargetAKT1
Source/Expression SystemHEK293 cells
Molecular Weight62kDa
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
ConcentrationPurified Ab WITHOUT BSA and Azide at 1.0mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Oncogene AKT1; PKB; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Proto-oncogene c-Akt; RAC Alpha; RAC Serine/Threonine Protein Kinase; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol

Available Sizes

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100 μg
$ 660.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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