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Description
Images & Validation
−| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 Kappa |
| Clone No. | X1 |
| Immunogen | AKT2 (AAA58364, 100 a.a. ~ 189 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Protein Sequence | MRAIQMVANSLKQRAPGEDPMDYKCGSPSDSSTTEEMEVAVSKARAKVTMNDFDYLKLLGKGTFGKVILVREKATGRYYAMKILRKEVII |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | In 1x PBS, pH 7.4 |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |

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AKT2 monoclonal antibody (M06), clone X1. Western Blot analysis of AKT2 expression in NIH/3T3.

AKT2 monoclonal antibody (M06), clone X1 Western Blot analysis of AKT2 expression in Jurkat.

AKT2 monoclonal antibody (M06), clone X1. Western Blot analysis of AKT2 expression in PC-12.

AKT2 monoclonal antibody (M06), clone X1. Western Blot analysis of AKT2 expression in Raw 264.7.

Detection limit for recombinant GST tagged AKT2 is approximately 1 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to AKT2 on HeLa cell. [antibody concentration 10 ug/ml]

Immunoperoxidase of monoclonal antibody to AKT2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]

Western Blot detection against Immunogen (35.53 KDa).
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Protocol Information
AKT2 monoclonal antibody (M06), clone X1 (orb2296222)
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