Aldehyde Dehydrogenase 1A1 Antibody / ALDH1A1

SKU: orb2635093

Description

ALDH1A1 belongs to the ALDH enzymes, a family of evolutionarily conserved enzymes comprised of 19 isoforms that are localized in the cytoplasm, mitochondria or nucleus. ALDH1A1 is predominantly expressed in the epithelium of testis, brain, eye, liver, kidney, as well as neural and hematopoietic stem cells. Reportedly, high ALDH1A1 expression is found in solitary fibrous tumor (SFT) and hemangiopericytoma (HPC), compared to meningiomas and synovial sarcomas. In combination with CD34, ALDH1A1 may be useful for the differentiation among SFT, HPC, meningioma, and synovial sarcoma.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Aldehyde Dehydrogenase 1A1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: ALDH1A1/4793
• Immunogen: Recombinant full-length human ALDH1A1 protein was used as the immunogen for the Aldehyde Dehydrogenase 1A1 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This Aldehyde Dehydrogenase 1A1 antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human sarcoma tissue with Aldehyde Dehydrogenase 1A1 antibody (clone ALDH1A1/4793) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon carcinoma tissue with Aldehyde Dehydrogenase 1A1 antibody (clone ALDH1A1/4793). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon carcinoma tissue with Aldehyde Dehydrogenase 1A1 antibody (clone ALDH1A1/4793). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast carcinoma tissue with Aldehyde Dehydrogenase 1A1 antibody (clone ALDH1A1/4793) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human HepG2 cell lysate using Aldehyde Dehydrogenase 1A1 antibody (clone ALDH1A1/4793). Expected molecular weight ~55 kDa.

SDS-PAGE analysis of purified, BSA-free Aldehyde Dehydrogenase 1A1 antibody (clone ALDH1A1/4793) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Aldehyde Dehydrogenase 1A1 antibody (clone ALDH1A1/4793). These results demonstrate the foremost specificity of the ALDH1A1/4793 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Quick Database Links

UniProt

P00352