Alkaline Phosphatase Conjugated Affinity Purified anti-Donkey IgG [H&L] [Goat]

SKU: orb1675047

Description

Alkaline Phosphatase Conjugated Affinity Purified anti-Donkey IgG [H&L] [Goat]

Images & Validation

• Tested Applications: DOT, ELISA, IHC-P, WB
• Reactivity: Donkey
• Application Notes:
  This product has been assayed against 1.0 µg of Donkey IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:16,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Immunogen: Donkey IgG whole molecule
• Conjugation: AP

Storage & Handling

• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
• Buffer/Preservatives: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
• Concentration: 0.59 mg/ml (by UV absorbance at 280 nm)
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only