Alpha-1-Antichymotrypsin Antibody / AACT / SERPINA3

SKU: orb385969

Description

Alpha 1-antichymotrypsin, or AACT, is a plasma protease inhibitor synthesized in the liver as a single glycopeptide chain. In human, the normal serum level of AACT is about one-tenth that of alpha 1-antitrypsin (AAT), with which it shares nucleic acid and protein sequence homology. Both are major acute phase reactants; their concentrations in plasma increase in response to trauma, surgery and infection. Elevated levels of AACT are widely, but not universally, reported in the cerebrospinal fluid and plasma of AD patients. Prostate-specific antigen (PSA) and its SDS-stable complex with AACT are in widespread use as markers for the diagnosis of prostate cancer. AACT deficiency may also be a possible cause of chronic liver disease. AACT antibody reacts with histiocytes and histiocytic neoplasms. It is widely used to identify histiocytes and tumors derived from them. Acinar tumors of the pancreas and salivary gland may also exhibit AACT positivity.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Immunology,Epigenetics

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The stated application concentrations are suggested starting amounts. Titration of the Alpha-1-Antichymotrypsin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: AACT/1451
• Immunogen: A human partial recombinant protein was used as the immunogen for this Alpha-1-Antichymotrypsin antibody.
• Purity: Protein G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store the Alpha-1-Antichymotrypsin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This Alpha-1-Antichymotrypsin antibody is available for research use only.
• Disclaimer: For research use only

IHC testing of FFPE human pancreas with Alpha-1-Antichymotrypsin antibody (clone AACT/1451). Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free Alpha-1-Antichymotrypsin Antibody (clone AACT/1451). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P01011