alpha 5 beta 1 Integrin Antibody [M200 (Volociximab)], Rabbit IgG - Research Grade Biosimilar

SKU: orb1671991

This product can be custom made

Description

Research Area

,Immunology,Cancer,Signal Transduction,Other

Images & Validation

−

Item 1 of 2

Tested Applications	Blocking, ELISA, FC, IF, IHC
Reactivity	Human

Key Properties

−

Antibody Type	Primary Antibody
Clonality	Recombinant
Isotype	IgG kappa
Clone No.	M200 (Volociximab)
Immunogen	Heterodimeric α5β1Fc fusion protein
Target	ITGA5
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	batch dependent
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

Fibronectin receptor, CD49 antigen-like family member E, Integrin alpha-F, VLA-5, CD49e.

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

alpha 5 beta 1 Integrin Antibody [M200 (Volociximab)], Rabbit IgG - Research Grade Biosimilar

Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volocixim449). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of M200 (orb1671991) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671991- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.

Flow-cytometry using the anti-alpha 5 beta 1 Integrin M200 (Volocixim449) PBMC's (A) and U93 cells (B) were stained with unimmunized r antibody (black line) or the rmeric version of M200 (Volocixim91 - blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.