Alpha Actinin 4 Recombinant Rabbit Monoclonal Antibody

SKU: orb1152016

Description

Alpha Actinin 4 Recombinant Rabbit Monoclonal Antibody

Research Area

Product Categories/Products/Antibodies,Response to hypoxia,Cell adhesion,Extracellular matrix,Hypoxia,Cancer,Actin Crosslinking

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:200-1000, IHC-P=1:20-1:100, IHC-F=1:400-800, ICC/IF=1:20-1:100, IF=1:20-1:100
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: A12H9
• Immunogen: KLH conjugated synthetic peptide derived from human alpha Actinin 4
• Target: ACTN4
• Molecular Weight: 105 kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

actinin 4; Actinin alpha 4; Actinin alpha-4; Alpha-actinin-4; α-Actinin-4; αActinin-4; actinin4; ACTN 4; ACTN4; ACTN4_HUMAN; DKFZp686K23158; F actin cross linking protein; F-actin cross-linking protein; Focal segmental glomerulosclerosis 1; FSGS 1; FSGS; FSGS1; Non muscle alpha actinin 4; Non-muscle alpha-actinin 4.

Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1152016.

Sample: Lane 1: Mouse Skin tissue lysates, Lane 2: Mouse Uterus tissue lysates, Lane 3: Human HeLa cell lysates, Lane 4: Human Jurkat cell lysates, Lane 5: Human U251 cell lysates, Primary: Anti-alpha Actinin 4 (orb1152016) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 105 kDa, Observed band size: 108 kDa.

Tissue: Human colon, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1152016.

Quick Database Links

Gene Symbol

ACTN4

UniProt

O43707