Alpha B Crystallin Antibody / CRYAB

SKU: orb1826002

Description

Crystallins are the major proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into Alpha, beta and gamma families, and the beta- and gamma-crystallins also compose a superfamily. Crystallins usually contain seven distinct protein regions, including four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha-crystallins consist of three gene products, AlphaA-, AlphaB- and AlphaC-crystallin, which are members of the small heat shock protein family (HSP 20). Alpha-crystallins act as molecular chaperones by holding denatured proteins in large soluble aggregates. However, unlike other molecular chaperones, Alpha-crystallins do not renature these proteins. Expression of AlphaA-crystallin is restricted to the lens and defects of this gene cause the development of autosomal dominant congenital cataracts (ADCC). The human AlphaB-crystallin gene product is expressed in many tissues, including lens, heart and skeletal muscle. Elevated expression of AlphaB-crystallin is associated with many neurological diseases, and a missense mutation in this gene has co-segregated in a family with a Desmin-related myopathy.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2, kappa
• Clone No.: CRYAB/4662
• Immunogen: Recombinant human full-length CRYAB protein was used as the immunogen for the CRYAB antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot the CRYAB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This CRYAB antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human skeletal muscle tissue with Crystallin Alpha B antibody (clone CRYAB/4662). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human kidney carcinoma tissue with Crystallin Alpha B antibody (clone CRYAB/4662). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P02511