Alpha B Crystallin Antibody: RPE

SKU: orb151552

Description

Rabbit polyclonal to Alpha B Crystallin (RPE). The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with Hsp25 and Hsp27. Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its phosphorylated state and may serve a structural control function and play a role in protein maintenance. In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases. Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions..

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cardiovascular Disease,Neuroscience,Epigenetics,Cancer | Heat Shock | Cell Signaling | Protein Trafficking | Chaperone Proteins | Neuroscience

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:5000), ICC/IF (1:120)
• Reactivity: Bovine, Gallus, Human, Mouse, Rat
• Application Notes:
  A 1:5000 dilution of SPC-126 was sufficient for detection of alpha B crystallin in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to human alpha B crystallin conjugated to KLH
• Target: Alpha B Crystallin
• Molecular Weight: 22kDa
• Conjugation: RPE

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AACRYA antibody, Alpha crystallin B chain antibody, CRYA2 antibody, CRYAB antibody, CTPP2 antibody, HSPB5 antibody, NY REN 27 antigen antibody

Quick Database Links

Gene Symbol

Alpha B Crystallin

UniProt

P02511

NCBI Gene ID

1410

RefSeq (Protein):NP_001876.1