Alpha Synuclein Antibody (pSer129): PerCP

SKU: orb414140

Description

Rabbit polyclonal antibody against Alpha Synuclein pSer129 conjugated to PerCP

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Neuroscience,Signaling Pathways,Product Categories/Products/Antibodies/Primary Antibodies,Signaling Pathways/Alzheimer's,Epigenetics,Neuroscience | Neurodegeneration | Alzheimer's Disease | Tangles & Tau | Neuroscience | Neurodegeneration | Parkinson's Disease | Synuclein | Neuroscience | Neurodegeneration | Multiple System Atrophy

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, WB
• Dilution Range: WB (1:1000); ICC/IF (1:500)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:1000 dilution of SPC-742 was sufficient for detection of Alpha Synuclein pSer129 in 15 µg of human brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of Human Alpha Synuclein pSer129 (124-134 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
• Target: Alpha Synuclein pSer129
• Molecular Weight: 100, 75, 45, 15 kDa
• Conjugation: PerCP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 0.5mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Phospho anti-alpha Synuclein (S129) antibody, Alpha-synuclein (phospho S129) antibody, alpha Synuclein (phospho Ser129) antibody, alpha-Synuclein Phospho Ser129 antibody, phospho-α-Synuclein (Ser129) antibody, Alpha synuclein antibody, Alpha synuclein antibody phospho Serine 129, Alpha synuclein antibody phospho Ser 129, Alpha synuclein antibody pSerine 129, Alpha synuclein antibody pSer 129, Alpha synuclein antibody phosphoSer 129, Alpha-synuclein antibody, Alpha-synuclein, isoform NACP140 antibody, alphaSYN antibody, NACP antibody, Non A beta component of AD amyloid antibody, Non A4 component of amyloid antibody, Non A4 component of amyloid precursor antibody, Non-A beta component of AD amyloid antibody, Non-A-beta component of alzheimers disease amyloid , precursor of antibody, Non-A4 component of amyloid precursor antibody, Non-A4 component of amyloid, precursor of antibody, PARK 1 antibody, PARK 4 antibody, PARK1 antibody, PARK4 antibody, Parkinson disease (autosomal dominant, Lewy body) 4 antibody, Parkinson disease familial 1 antibody, SNCA antibody, alpha (non A4 component of amyloid precursor) antibody, SYN antibody, Synuclein alpha antibody, Synuclein alpha 140 antibody, Synuclein, alpha (non A4 component of amyloid precursor) antibody, SYUA_HUMAN antibody

Immunohistochemistry analysis using Rabbit Anti-Alpha Synuclein (pSer129) Polyclonal Antibody. Tissue: Free floating brain sections. Species: Mouse. Fixation: PFA. Primary Antibody: Rabbit Anti-Alpha Synuclein (pSer129) Polyclonal Antibody at 1:500 for overnight at 4°C with gentle agitation. Counterstain: Hoechst. Magnification: 63X. A) Right hemisphere (striatum) injected with alpha synuclein AAV vector. B) Control. Alpha synuclein streaks are visible at injection site, but not control, 4 months after injection.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Alpha Synuclein (pSer129) Polyclonal Antibody. Species: Mouse. Primary Antibody: Rabbit Anti-Alpha Synuclein (pSer129) Polyclonal Antibody. Phospho serine 129 antibody was used to detect phosphorylated alpha synuclein in primary mouse hippocampal neurons treated with 100 nM sonicated mouse alpha synuclein PFFs (A). Phosphorylated alpha synuclein was visible in perinucleus and neurites compared to untreated control (B).

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody. Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate at 4 μg/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody at 1:500 for 24 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); Hoechst (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X.

Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa Alpha Synuclein pSer129 protein using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Human Alpha Synuclein Monomer (0.5 μg). Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band (s): 100, 75, 45, 30, 16 kDa.

Immunohistochemistry analysis using Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody. Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Quick Database Links

Gene Symbol

Alpha Synuclein pSer129

UniProt

P37840

NCBI Gene ID

6622

RefSeq (Protein):NP_000336.1