Alpha Synuclein Antibody (pSer129): HRP

SKU: orb612727

Description

Rabbit monoclonal antibody against Alpha Synuclein (Phospho-Ser129) conjugated to HRP

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Neuroscience,Signaling Pathways,Product Categories/Products/Antibodies/Primary Antibodies,Signaling Pathways/Alzheimer's,Epigenetics,Neuroscience | Neurodegeneration | Alzheimer's Disease | Tangles & Tau | Neuroscience | Neurodegeneration | Parkinson's Disease | Synuclein | Neuroscience | Neurodegeneration | Multiple System Atrophy

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB (1:500)
• Reactivity: Human, Mouse
• Application Notes:
  A 1:500 dilution of SMC-600 was sufficient for detection of Alpha Synuclein pSer129 in 10 µg of Mouse Brain by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: J18
• Immunogen: Human alpha synuclein AA 124-134: AYEMP-pS-EEGYQ-Cys
• Target: Alpha Synuclein pSer129
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Phosphorylated alpha synuclein antibody, Phospho anti-alpha Synuclein (S129) antibody, Alpha-synuclein (phospho S129) antibody, alpha Synuclein (phospho Ser129) antibody, alpha-Synuclein Phospho Ser129 antibody, phospho-α-Synuclein (Ser129) antibody, Alpha synuclein antibody, Alpha synuclein antibody phospho Serine 129, Alpha synuclein antibody phospho Ser 129, Alpha synuclein antibody pSerine 129, Alpha synuclein antibody pSer 129, Alpha synuclein antibody phosphoSer 129, Alpha-synuclein antibody, Alpha-synuclein, isoform NACP140 antibody, alphaSYN antibody, NACP antibody, Non A beta component of AD amyloid antibody, Non A4 component of amyloid antibody, Non A4 component of amyloid precursor antibody, Non-A beta component of AD amyloid antibody, Non-A-beta component of alzheimers disease amyloid , precursor of antibody, Non-A4 component of amyloid precursor antibody, Non-A4 component of amyloid, precursor of antibody, PARK 1 antibody, PARK 4 antibody, PARK1 antibody, PARK4 antibody, Parkinson disease (autosomal dominant, Lewy body) 4 antibody, Parkinson disease familial 1 antibody, SNCA antibody, alpha (non A4 component of amyloid precursor) antibody, SYN antibody, Synuclein alpha antibody, Synuclein alpha 140 antibody, Synuclein, alpha (non A4 component of amyloid precursor) antibody, SYUA_HUMAN antibody

Immunohistochemistry analysis using Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody, Clone J18. Tissue: Brain. Species: Mouse. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody at 1:10000. Secondary Antibody: anti-rabbit HRP. C57/BL6 mice were injected with 5 ug sonicated mouse recombinant alpha synuclein PFFs at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) contralateral cortex. (B) ipsilateral cortex. (C) contralateral striatum. (D) ipsilateral striatum.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Alpha Synuclein (pSer129) Monoclonal Antibody, Clone J18. Tissue: iPSC-derived neurons. Species: Human. Primary Antibody: Rabbit Anti-Alpha Synuclein (pSer129) Monoclonal Antibody at 1:1000 for O/N at 4°C. Secondary Antibody: Anti-Rabbit: A488 at 1:1000 for 1 hour at RT. Magnification: 40X. Nuclear stain: Hoechst- 20 min, RT (blue). Actin stain: Phalloidin-647- 20 min, RT (magenta). 4K cells per well. A) negative control; no fibrils added to well. B) 7 days after addition of active recombinant human pre-formed fibrils (Type 1).

Immunohistochemistry analysis using Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody, Clone J18. Tissue: Brain. Species: Mouse. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody at 1:10000. Secondary Antibody: anti-rabbit HRP. C57/BL6 mice were injected with sonicated recombinant mouse alpha synuclein monomers or fibrils at 8 weeks of age. Mice were unilaterally injected in the dorsal striatum (bregma AP + 0.2 mm, L +/1 2.0 mm, V - 3.0 mm) and sacrificed 30 days post-injection. (A) 1.25 uL mouse alpha synuclein monomers. (B) 2.5 uL mouse alpha synuclein monomers. (C) 2.5 ug alpha synuclein PFFs. (D) 5 ug alpha synuclein PFFs. Inset: PBS (negative control).

Dot Blot analysis using Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody, Clone J18. Tissue: alpha synuclein peptide. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Phospho peptide sequence: AYEMP-pS-EEGYQ. Non-phospho peptide sequence: AYEMPSEEGYQ. This sequence is the same for human, mouse, and rat.

Western Blot analysis of Human Alpha Synuclein showing detection of Alpha Synuclein pSer129 protein using Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody, Clone J18. Lane 1: MW ladder. Lane 2: 0.5 ug human alpha synuclein monomer. Lane 3: 2 ug human alpha synuclein monomer. Lane 4: 0.5 ug human alpha synuclein PFFs. Lane 5: 2 ug human alpha synuclein PFFs. Lane 6: 15 ug human Parkinson's Disease brain lysate. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.

Western Blot analysis of Mouse Brain showing detection of Alpha Synuclein pSer129 protein using Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody, Clone J18. Lane 1: MW ladder. Lane 2: Mouse brain. Load: 15 ug. Block: 5% BSA in TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein pSer129 Monoclonal Antibody at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.

Immunocytochemistry / Immunofluorescence analysis of human iPSC-derived neurons treated with 2.5μg ATTO 594 labeled type I alpha-synuclein pre-formed fibrils for up to 14 days. Cells seeded at 8k cells per well. Green: mouse anti-alpha synuclein (pSer129) monoclonal antibody 1:5000; Red: alpha-synuclein PFFs; Pink: actin; Blue: Hoechst / DNA.

Quick Database Links

Gene Symbol

Alpha Synuclein pSer129

UniProt

P37840

NCBI Gene ID

6622

RefSeq (Protein):NP_000336.1