AMBRA1 Antibody: HRP

SKU: orb384101

Description

Rabbit polyclonal antibody against AMBRA1 conjugated to HRP.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Neuroscience,Signaling Pathways,Product Categories/Products/Antibodies/Primary Antibodies,Signaling Pathways/Autophagic,Epigenetics,Developmental Biology,Cancer | Autophagy| Neuroscience | Cell Signaling | Metabolism | Mitochondrial Metabolism | Cardiovascular System | Heart

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human, Rat
• Application Notes:
  A 1:1000 dilution of SPC-644 was sufficient for detection of AMBRA1 in 15 µg of Rat Brain Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of Human AMBRA1 (aa. 200-300)
• Target: AMBRA1
• Molecular Weight: 142 kDa
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AMBRA1 antibody, Activating molecule in BECN1-regulated autophagy protein 1 antibody, KIAA1736 antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AMBRA1 Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-AMBRA1 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Autophagosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) AMBRA1 Antibody (D) Composite.

Western blot analysis of Rat Brain cell lysates showing detection of 142.5 kDa AMBRA1 protein using Rabbit Anti-AMBRA1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AMBRA1 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 142.5 kDa. Other Band (s): 50 kDa.

Quick Database Links

Gene Symbol

AMBRA1

UniProt

Q9C0C7

NCBI Gene ID

55626

RefSeq (Protein):NP_001254711.1