Annexin A1 Antibody

SKU: orb2643347

Description

The ANXA1 gene belongs to the annexin family, and contains 4 annexin repeats. A pair of annexin repeats may form one binding site for calcium and a phospholipid. ANXA1 promotes membrane fusion and is involved in exocytosis. The gene for ANXA1 is upregulated in hairy cell leukemia (HCL), and its protein expression is specific for HCL. Detection of ANXA1 provides a simple, highly sensitive and specific assay for diagnosing HCL. Annexin A1 has also been found to be protective against DNA damage induced by heat in breast cancer cells, suggesting it is involved in tumor suppressive and protective activities, and also is associated with treatment resistance.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: ANXA1/3566
• Immunogen: Recombinant full-length human Annexin A1 protein was used as the immunogen for the Annexin A1 antibody.
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This Annexin A1 antibody is available for research use only.
• Disclaimer: For research use only

Immunofluorescent staining of PFA-fixed human HeLa cells with Annexin A1 antibody (green, clone ANXA1/3566) and Reddot nuclear stain (red).

IHC staining of FFPE human lymph node with Annexin A1 antibody (clone ANXA1/3566). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human lymph node with Annexin A1 antibody (clone ANXA1/3566). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human colon carcinoma with Annexin A1 antibody (clone ANXA1/3566). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human prostate carcinoma with Annexin A1 antibody (clone ANXA1/3566). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human A549 cell lysate with Annexin A1 antibody (clone ANXA1/3566). Predicted molecular weight ~38 kDa.

Flow cytometry testing of PFA-fixed human HeLa cells with Annexin A1 antibody (clone ANXA1/3566); Red=isotype control, Blue= Annexin A1 antibody.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Annexin A1 antibody (clone ANXA1/3566). These results demonstrate the foremost specificity of the ANXA1/3566 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Annexin A1 antibody (clone ANXA1/3566) as confirmation of integrity and purity.

Quick Database Links

UniProt

P04083