Annexin V-FITC Apoptosis Assay Kit

SKU: orb1409916

Description

Detect apoptosis and membrane permability with the Annexin V-FITC Apoptosis Assay Kit. This in vitro apoptosis assay employs the green fluorescent Annexin V-FITC reagent to label exposed phosphatidylserine in cultured cell samples. Detect membrane compromised cells, a trademark of late apoptosis or cell necrosis, with the live/dead stain, propidium iodide. Analyze the fluorescent signals by flow cytometry.

Research Area

,Product Categories/ELISA Kits

Images & Validation

• Application Notes:
  1. Prepare samples and controls, 2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH2O., 3. Reconstitute FLICA with 50 µL DMSO., 4. Dilute FLICA 1:5 by adding 200 µL PBS., 5. Add diluted FLICA to each sample at 1:30-1:60 (e.g. spike at 1:30 by adding 10 µL to 290 µL cultured cells)., 6. Incubate approximately 1 hour., 7. Wash and spin cells three times., 8. If desired, label with additional stains, such as Hoechst, DAPI, or an antibody., 9. If desired, fix cells., 10. Analyze with a fluorescence microscope or flow cytometer. FLICA 660 excites at 660 nm and emits at 680-690 nm.

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Key Properties

• Target: Apoptosis, Exposed phosphatidyl serine
• Sample Types: Cell culture

Storage & Handling

• Storage: 2-8°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Apoptosis, Exposed phosphatidyl serine