Anti-ABCG1 Antibody

SKU: orb214917

Description

Rabbit polyclonal antibody to ABCG1

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1-500-1-1000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ABCG1. The exact sequence is proprietary.
• Target: ABCG1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ABC8 antibody, anti WHT1 antibody, anti ATP-binding cassette sub-family G member 1 antibody, anti ATP-binding cassette transporter 8 antibody, anti White protein homolog antibody

Western blot analysis of ABCG1 expression in MCF7 (A), Hela (B), PC12 (C), RAW264.7 (D), SHSY5Y (E), THP1 (F) whole cell lysates. (Predicted band size: 75 kD; Observed band size: 76 kD)

Immunofluorescent analysis of ABCG1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

ABCG1

UniProt

P45844, Q64343

NCBI Gene ID

9619, 11307

Kato, Kosuke et al. Opposite effects of tumor protein D (TPD) 52 and TPD54 on oral squamous cell carcinoma cells Int J Oncol, 50, 1634-1646 (2017)

PubMed: 28339026